Effects of Local Anthropogenic Changes on Potential Malaria Vector Anopheles hyrcanus and West Nile Virus Vector Culex modestus, Camargue, France

Sixty years of environmental modifications have led to strong and rapid effects on the abundance of vector populations

La prothèse inversée d'épaule delta III dans le cadre de ruptures massives et irréparables de la coiffe des rotateurs avec ou sans omarthrose

PARIS6-Bibl. St Antoine CHU (751122104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Apport de la chirurgie assistée par ordinateur dans l'arthroplastie totale du genou (à propos de 47 cas)

PARIS6-Bibl.Pitié-Salpêtrie (751132101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

ETUDE D'UNE SERIE DE 9 CAS DE NEUROLYSE CHIRURGICALE DU NERF SUPRA-SCAPULAIRE

PARIS6-Bibl. St Antoine CHU (751122104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

ETUDE D'UN BIOMATERIAU HYDRIDE DANS UN MODELE DE GRANDE RESECTION OSSEUSE CHEZ LE LAPIN

PARIS-BIUM (751062103) / SudocCentre Technique Livre Ens. Sup. (774682301) / SudocSudocFranceF

ETUDE MORPHOMETRIQUE DE L'EXTREMITE SUPERIEURE DE L'HUMERUS APRES ACQUISITION TOMODENSITOMETRIQUE

PARIS7-Xavier Bichat (751182101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Clearing method for 3-dimensional immunofluorescence of osteoarthritic subchondral human bone reveals peripheral cholinergic nerves

International audienceThe cholinergic system plays a major anti-inflammatory role in many diseases through acetylcholine (Ach) release after vagus nerve stimulation. Osteoarthritis (OA) is associated with local low-grade inflammation, but the regulatory mechanisms are unclear. Local Ach release could have anti-inflammatory activity since articular cells express Ach receptors involved in inflammatory responses. Using the 3DISCO clearing protocol that allows whole-sample 3-dimensional (3D) analysis, we cleared human OA cartilage-subchondral bone samples to search for cholinergic nerve fibres able to produce Ach locally. We analysed 3 plugs of knee cartilage and subchondral bone from 3 OA patients undergoing arthroplasty. We found no nerves in the superficial and intermediate articular cartilage layers, as evidenced by the lack of Peripherin staining (a peripheral nerves marker). Conversely, peripheral nerves were found in the deepest layer of cartilage and in subchondral bone. Some nerves in the subchondral bone samples were cholinergic because they coexpressed peripherin and choline acetyltransferase (ChAT), a specific marker of cholinergic nerves. However, no cholinergic nerves were found in the cartilage layers. It is therefore feasible to clear human bone to perform 3D immunofluorescence. Human OA subchondral bone is innervated by cholinergic fibres, which may regulate local inflammation through local Ach release

Heterolytic Splitting of H 2 and CH 4 on γ-Alumina as a Structural Probe for Defect Sites

International audienc

CH3-ReO3 on gamma-Al2O3: understanding its structure, initiation, and reactivity in olefin metathesis

Me-ReO3 on gamma-alumina: understanding the structure, the initiation and thereactivity of a highly active olefin metathesis catalyst Heterolytic splitting of the C-H bond of the methyl group of CH3ReO3 on AlsO reactive sites of alumina as a way to generate the active site of CH3ReO3 supported on gamma-Al203